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pei max 40 kda  (Kyfora Bio)


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    Kyfora Bio pei max 40 kda
    Pei Max 40 Kda, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 96/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pei+max+40+kda/Polyethylenimine%2C+Linear/us12487152-163-6-9
    Average 96 stars, based on 94 article reviews
    pei max 40 kda - by Bioz Stars, 2026-09
    96/100 stars

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    Variant Assay:

    Article Title: Efficient and inexpensive transient expression of multispecific multivalent antibodies in Expi293 cells
    Article Snippet: .. Instead, a more easily dissolved variant is used: PEI Max 40 kDa (Polysciences). ..

    Expressing:

    Article Title: Scalable method for producing transfection reagents
    Article Snippet: .. PEI solutions were made by dissolving PEI-MAX 40 kDa (PolySciences) in FreeStyleTM F17 Expression Media (ThermoFisher) at concentrations of 20, 40, and 80 μg/ml. ..

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids.
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Cell lines and tissue culture Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l−1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml−1 penicillin and 100 μg ml−1 streptomycin.

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l −1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml −1 penicillin and 100 μg ml −1 streptomycin.

    Transfection:

    Article Title: Cooperation Between Systemic IgG1 and Mucosal Dimeric IgA2 Monoclonal Anti-HIV Env Antibodies: Passive Immunization Protects Indian Rhesus Macaques Against Mucosal SHIV Challenges
    Article Snippet: .. Transfections were performed using PEI MAX 40 kDa (Polysciences – Brunschwig) as a transfection agent at a PEI : DNA ratio of 3:1 and 1 μg of total DNA/ml cell culture. .. Transfected cells were supplemented three days after transfection with Soy hydrolysate (Sigma) and glucose (Sigma) and cultured for an additional four days.

    Cell Culture:

    Article Title: Cooperation Between Systemic IgG1 and Mucosal Dimeric IgA2 Monoclonal Anti-HIV Env Antibodies: Passive Immunization Protects Indian Rhesus Macaques Against Mucosal SHIV Challenges
    Article Snippet: .. Transfections were performed using PEI MAX 40 kDa (Polysciences – Brunschwig) as a transfection agent at a PEI : DNA ratio of 3:1 and 1 μg of total DNA/ml cell culture. .. Transfected cells were supplemented three days after transfection with Soy hydrolysate (Sigma) and glucose (Sigma) and cultured for an additional four days.

    Incubation:

    Article Title: Methods and compositions for treating metastatic breast cancer and other cancers in the brain
    Article Snippet: 2 mL PEI-Max 40 KDa, linear (Polysciences) at 1 mg/mL and pH 7.1 was diluted separately in 42 mL room-temperature antibiotic and serum-free DMEM, Diluted DNA and diluted PEI were combined and incubated for 15 minutes at room temperature. .. 2 mL PEI-Max 40 KDa, linear (Polysciences) at 1 mg/mL and pH 7.1 was diluted separately in 42 mL room-temperature antibiotic and serum-free DMEM, Diluted DNA and diluted PEI were combined and incubated for 15 minutes at room temperature. ..

    Staining:

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids.
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Cell lines and tissue culture Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l−1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml−1 penicillin and 100 μg ml−1 streptomycin.

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l −1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml −1 penicillin and 100 μg ml −1 streptomycin.

    Magnetic Beads:

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids.
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Cell lines and tissue culture Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l−1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml−1 penicillin and 100 μg ml−1 streptomycin.

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l −1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml −1 penicillin and 100 μg ml −1 streptomycin.

    Protease Inhibitor:

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids.
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Cell lines and tissue culture Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l−1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml−1 penicillin and 100 μg ml−1 streptomycin.

    Article Title: Structural basis for the dynamic regulation of mTORC1 by amino acids
    Article Snippet: Reagents were obtained from the following sources: antibodies to S6K pT389 (9234), S6K (2708), MIOS (13557), WDR59 (53385), Sestrin2 (8487), MYC epitope tag (2276 and 2278), Flag epitope tag (14793), HA epitope tag (3724) and horseradish peroxidase-linked anti-rabbit secondary antibody (7074) were from Cell Signaling Technology; antibodies to SEH1L (ab218531) and DEPDC5 (ab213181 and ab185565) were from Abcam; antibodies to SEC13 (15397-1-AP), WDR24 (20778-1-AP) and KPTN (16094-1-AP) were from Proteintech; antibodies to Raptor (09-217) and Flag-M2 (F1804) used for preparing anti-Flag magnetic beads were from MilliporeSigma; and antibody to NPRL3 was from Novus Biologicals (NBP1-88447). .. InstantBlue Coomassie Protein Stain was from Abcam; anti-Flag-M2 affinity gels, ATP and amino acids were from MilliporeSigma; DMEM, Expi293 Expression Medium, inactivated fetal serum, Dynabeads M-270 epoxy, Dynabeads protein G and anti-HA magnetic beads were from Thermo Fisher Scientific; XtremeGene9, PhosSTOP and cOmplete Protease Inhibitor were from Roche; amino acid-deficient RPMI (R8999-03A and R9010-01) was from US Biologicals; and PEI MAX 40 kDa was from Polysciences. .. Adherent HEK293T cells were cultured in DMEM (Thermo Fisher Scientific) with 10% inactivated fetal serum (Thermo Fisher Scientific) and 4.5 g l −1 glucose containing 2 mM GlutaMAX (Thermo Fisher Scientific), 100 IU ml −1 penicillin and 100 μg ml −1 streptomycin.



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